Other Photographs 2007 2006 2005
Recent News
June 2009
- We received notice of award of funds from the Murdock Foundation (http://www.murdock-trust.org/) to purchase a Waters SYNAPT mass spectrometer.
June 2008
- ASMS(American Society for Mass Spectrometry) conference in Denver, Colorado. 56th ASMS Conference on Mass Spectrometry
September 2007
- Goodlett Group Retreat in Talaris
June 2007
- ASMS(American Society for Mass Spectrometry) conference in Indianapolis, Indiana. 55th ASMS Conference on Mass Spectrometry
October 2006
- The Goodlett Lab has announced an official collaboration with GeneBio through use of their MS searching engine Phenyx. Official Press Release.
May 2006
- The Goodlett Lab is hosting an open house on Friday, June 2. For more information and directions see the invitation.
March 2006
- A paper describing Pro-CrossLink has been published in Analytical Chemistry. To obtain the software, follow this link.
January 2006
- Lab meeting schedule posted here.
May 2005
UK based RASOR grant to develop proteomic technologies started May 2005. For more information see the RASOR website.
February 2005
Supported by a donation from Dell and Intel, we have purchased a 40 node cluster for running SEQUEST and the Sashimi trans-proteomic pipeline.
January 2005
From 6-9 December 2005 the Goodlett Lab is hosting the Third International conference on Electron Capture Dissociation and Allied Topics at Salish Lodge and Spa. Please stay tuned for more information.
Using funds from a grant to the UW from the W.M. Keck foundation, we have purchased an upgrade to the Bruker APEX III FTICRMS instrument located in our department that will allow electron capture dissociation (ECD) with infrared multi-photon dissociation (IRMPD) as well as collision induced dissociation of ions in quadrupoles located prior to the ICR cell.
December, 2004
The mass spectrometry center acquired the Applied Biosystems 4700 Proteomics Analyzer from the Murdock Trust Fund. It is a MALDI tandem time-of-flight mass spectrometer that provides a new level of productivity in high-resolution proteome analysis and complex systems biology.
RESEARCH OVERVIEW
We are a bioanalytical laboratory that develops separation science and mass spectrometric methods to solve biological problems of interest to us and our collaborators. In general we seek to use mass spectrometry to extract the maximum amount of information with a minimum of fractionation prior to MS analysis, which we refer to as Indolent Driven Science. Our primary tool is mass spectrometry and specifically Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS). We have upgraded an older Bruker APEX III FTICRMs instrument to allow electron capture dissociation (ECD) with infrared multi-photon dissociation (IRMPD) as well as collision induced dissociation of ions in quadrupoles located prior to the ICR cell. Additionally, we have implemented a simple version of Dynamic Range Expansion Applied to Mass Spectrometry (DREAMS) developed by the R.D. Smith laboratory on this instrument. We are currently pursuing data-independent analysis of complex mixtures using a protocol we refer to as Precursor Acquisition Independent From Ion Count (PAcIFIC; see Panchaud Anal Chem 2009) that is related to a type of data produced by a novel tandem mass spectrometer (see Wang RCMS 2007). Most recently we implemented an ion funnel on our Thermo LTQ-FT instrument (see Kelly et al. Mass Spectrom Rev 2009). Other work horse instruments in the laboratory include a Waters SYNAPT, a Thermo LTQ-Velos and a Thermo LTQ-Orbitrap all fitted with Waters NanoAcquity HPLCs. We are applying these and other technologies with our collaborators who are in the fields of microbiology, prostate cancer, urinary track disorders, HIV and fatigue, acute respiratory distress syndrome, and pediatric medulloblastomas. Finally, we are part of the University of Washington's Proteomics Resource**** (UWPR) consortium developed to aid proteomics research on campus.
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