University of Washington

Goodlett Laboratory

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Research Goals

We are a bioanalytical laboratory that develops separation science and mass spectrometric methods to solve biological problems of interest to us and our collaborators. Our primary tool is mass spectrometry and specifically Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS). We just received a Thermo LTQ-FT in April of 2004. In addition we have purchased an upgrade to the Bruker APEX III FTICRMS instrument located in our department that will allow electron capture dissociation (ECD) with infrared multi-photon dissociation (IRMPD) as well as collision induced dissociation of ions in quadrupoles located prior to the ICR cell. We are developing a novel form of parallel tandem mass spectrometer with Michrom Bioresources of Auburn, CA and testing a MALDI-ion trap-TOF-MS from Shimadzu Corp. We are applying our technologies in the fields of microbiology with Dr. Samuel Miller at the UW, lycopene and prostate cancer with Dr. Richard van Breemen of UIC, androgen receptor binding proteins and prostate cancer with Dr. Daniel Martin at the ISB, Acute respiratory distress syndrome with Dr. Lynn Schnapp of the UW, and pediatric medulloblastomas with Dr. Jim Olson of the FHCRC and Parkinson's disease with Dr. Jing Zhang of Harborview Medical Center. More about these projects can be in the research section.